APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human NUP153, corresponding to a region within C-terminal amino acids.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HNUP153 , N153 , NUP153 , nucleoporin 153 , Nup153
Cellular Localization
Nucleus,Nucleus membrane,Nuclear pore complex
Background
Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Database
Research Area
DATA IMAGES
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GTX00649 WB Image
WB analysis of HepG2 cell lysates using GTX00649 Nup153 antibody. The lane on the left was treated with blocking peptide.
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GTX00649 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX00649 Nup153 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX00649 WB Image
WB analysis of COLO205 cell lysate using GTX00649 Nup153 antibody.
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REFERENCE
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REVIEW
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