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Nup153 antibody

Anti-Nup153 antibody used in Western Blot (WB). GTX00649
Anti-Nup153 antibody used in Western Blot (WB). GTX00649
Anti-Nup153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00649

Cat No. GTX00649

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-3000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human NUP153, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HNUP153 , N153 , NUP153 , nucleoporin 153 , Nup153

Cellular Localization

Nucleus,Nucleus membrane,Nuclear pore complex

Background

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-Nup153 antibody used in Western Blot (WB). GTX00649

GTX00649 WB Image

WB analysis of COLO205 cell lysate using GTX00649 Nup153 antibody.

Anti-Nup153 antibody used in Western Blot (WB). GTX00649

GTX00649 WB Image

WB analysis of HepG2 cell lysates using GTX00649 Nup153 antibody. The lane on the left was treated with blocking peptide.

Anti-Nup153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00649

GTX00649 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00649 Nup153 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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