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Nup153 antibody

Anti-Nup153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132731
Anti-Nup153 antibody used in Western Blot (WB). GTX132731

Cat No. GTX132731

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

Positive Control

HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.23 mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nup153. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nucleoporin 153,HNUP153,N153

Cellular Localization

Nucleus

Background

Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES
Anti-Nup153 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132731

GTX132731 ICC/IF Image

Nup153 antibody detects Nup153 protein at nuclear envelope and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nup153 stained by Nup153 antibody (GTX132731) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Nup153 antibody used in Western Blot (WB). GTX132731

GTX132731 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nup153 antibody (GTX132731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 21858095

REFERENCE
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Package List Price ($)
$ 319
$ 169