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O-GlcNAc transferase antibody [GT2037]

Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813
Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813
Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813

Cat. No. GTX629813

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2037

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all O-GlcNAc transferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

mouse brain , 293T , HeLa , rat brain

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888161

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813

GTX629813 WB Image

O-GlcNAc transferase antibody [GT2037] detects O-GlcNAc transferase protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
O-GlcNAc transferase antibody [GT2037] (GTX629813) dilution: 1:1000

Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813

GTX629813 WB Image

O-GlcNAc transferase antibody [GT2037] detects O-GlcNAc transferase protein by western blot analysis.
A. 50 μg rat brain whole cell lysate/extract
7.5 % SDS-PAGE
O-GlcNAc transferase antibody [GT2037] (GTX629813) dilution: 1:500

Anti-O-GlcNAc transferase antibody [GT2037] used in Western Blot (WB). GTX629813

GTX629813 WB Image

O-GlcNAc transferase antibody [GT2037] detects O-GlcNAc transferase protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg HeLa
7.5 % SDS-PAGE
O-GlcNAc transferase antibody [GT2037] (GTX629813) dilution: 1:1000

REFERENCE

Application Reference

Skrajna A et al. Nucleic Acids Res 2020; 48 (17) : 9415-9432 Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations