APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , PC-12
Predict Reactivity
Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888160
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1
Cellular Localization
Cytoplasm (Potential) , Nucleus
Background
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812](/templates/default/js/lightbox/images/loading.gif "Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812")
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GTX629812 ICC/IF Image
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] (GTX629812) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX629812 IHC-P Image
O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at cytoplasm of islet by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.O-GlcNAc transferase stained by O-GlcNAc transferase antibody [GT678] (GTX629812) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629812 WB Image
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
O-GlcNAc transferase antibody [GT6G8] (GTX629812) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629812 WB Image
O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
O-GlcNAc transferase antibody [GT6G8] (GTX629812) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for O-GlcNAc transferase antibody [GT678] (GTX629812). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for O-GlcNAc transferase antibody [GT678] (GTX629812). Be the first to share your experience with this product.
![Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_IFA_18121411_399.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_20220401_IHC-P_M_22041900_912.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_WB_M_brain_18121411_136.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/small/GTX629812/GTX629812_41477_WB_R_18121411_816.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_IFA_18121411_399.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_20220401_IHC-P_M_22041900_912.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in IHC (Paraffin sections) (IHC-P). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_WB_M_brain_18121411_136.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")
![Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812](/upload/website/prouct_img/normal/GTX629812/GTX629812_41477_WB_R_18121411_816.jpg "Anti-O-GlcNAc transferase antibody [GT678] used in Western Blot (WB). GTX629812")