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O-GlcNAc transferase antibody [HL1641]

Recombinant antibody KO/KD Orthogonal
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

Cat. No. GTX637222

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1641

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all O-GlcNAc transferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Nucleus,Cytoplasm,Membrane,Cell membrane

Background

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222

GTX637222 IHC-P Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222

GTX637222 IHC-P Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-O-GlcNAc transferase antibody [HL1641] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637222

GTX637222 ICC/IF Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Su WC et al. In Vivo 2023; 37 (6) : 2524-2532 Thiamet G as a Potential Treatment for Polycystic Kidney Disease.
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations