APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1
Cellular Localization
Nucleus,Cytoplasm,Membrane,Cell membrane
Background
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/templates/default/js/lightbox/images/loading.gif "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
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GTX637223 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637223 WB Image
Various whole cell extracts (30 Âμg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637223 IHC-P Image
O-GlcNAc transferase antibody [HL1642] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637223 ICC/IF Image
O-GlcNAc transferase antibody [HL1642] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637223 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1642] (GTX637223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for O-GlcNAc transferase antibody [HL1642] (GTX637223). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for O-GlcNAc transferase antibody [HL1642] (GTX637223). Be the first to share your experience with this product.
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/small/GTX637223/GTX637223_T-44746_20220826_WB_shRNA_watermark_22083119_528.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/small/GTX637223/GTX637223_T-44746_20220805_WB_M_R_22080923_335.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in IHC (Paraffin sections) (IHC-P). GTX637223](/upload/website/prouct_img/small/GTX637223/GTX637223_T-44746_20220819_IHC-P_M_22083119_438.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in IHC (Paraffin sections) (IHC-P). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637223](/upload/website/prouct_img/small/GTX637223/GTX637223_T-44746_20220916_ICC_IF_22102723_913.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/small/GTX637223/GTX637223_T-44746_20220722_WB_22072519_924.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220826_WB_shRNA_watermark_22083119_528.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220805_WB_M_R_22080923_335.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in IHC (Paraffin sections) (IHC-P). GTX637223](/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220819_IHC-P_M_22083119_438.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in IHC (Paraffin sections) (IHC-P). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637223](/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220916_ICC_IF_22102723_913.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637223")
![Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223](/upload/website/prouct_img/normal/GTX637223/GTX637223_T-44746_20220722_WB_22072519_924.webp "Anti-O-GlcNAc transferase antibody [HL1642] used in Western Blot (WB). GTX637223")