APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.05-0.2μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89131-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
Predict Reactivity
Mouse, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1; NP_858059.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1
Cellular Localization
Nucleus , Cytoplasm , Membrane , Cell membrane
Background
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX89131 IHC-P Image
IHC-P analysis of human cortex using GTX89131 O-GlcNAc transferase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89131 WB Image
WB analysis of rat pancreas lysate using GTX89131 O-GlcNAc transferase antibody, Internal.
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for O-GlcNAc transferase antibody, Internal (GTX89131). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for O-GlcNAc transferase antibody, Internal (GTX89131). Be the first to share your experience with this product.
