O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution:
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX110136 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX110136 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:1000.

O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Immunohistochemical analysis (whole mount) of zebrafish embryo, using O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) at 1:200 dilution.