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O-GlcNAc transferase antibody [N1N2], N-term

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136
Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136

Cat. No. GTX110136

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all O-GlcNAc transferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

Mouse brain , HeLa , PC-12 , Rat2

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human O-GlcNAc transferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951058

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX110136

GTX110136 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) at 1:200 dilution.

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136

GTX110136 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX110136 diluted at 1:1000

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110136

GTX110136 IHC-P Image

O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110136

GTX110136 IHC-P Image

O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) dilution:
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110136

GTX110136 ICC/IF Image

O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136

GTX110136 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [N1N2], N-term (GTX110136) diluted at 1:1000.

Anti-O-GlcNAc transferase antibody [N1N2], N-term used in Western Blot (WB). GTX110136

GTX110136 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX110136 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169