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O-GlcNAc transferase antibody

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939
Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109939
Anti-O-GlcNAc transferase antibody used in Immunoprecipitation (IP). GTX109939
Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939
Anti-O-GlcNAc transferase antibody used in IHC (Paraffin sections) (IHC-P). GTX109939
Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109939
Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939

Cat No. GTX109939

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , PC-12

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

O-Linked N-Acetylglucosamine (Glcnac) Transferase,Hincut-1,Hrnt1,Mrx106,O-Glcnac,Ogt1,Ogt

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939

GTX109939 WB Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody (GTX109939) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109939

GTX109939 ICC/IF Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-O-GlcNAc transferase antibody used in Immunoprecipitation (IP). GTX109939

GTX109939 IP Image

Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody (GTX109939).
Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939

GTX109939 WB Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
5% SDS-PAGE
O-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody used in IHC (Paraffin sections) (IHC-P). GTX109939

GTX109939 IHC-P Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109939

GTX109939 ICC/IF Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939

GTX109939 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (GTX109939) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169