O-GlcNAc transferase antibody

Name: O-GlcNAc transferase antibody
Brand: GeneTex
SKU: GTX109939
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX109939

Anti-O-GlcNAc transferase antibody used in Immunoprecipitation (IP). GTX109939

Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109939

Anti-O-GlcNAc transferase antibody used in IHC (Paraffin sections) (IHC-P). GTX109939

Cat. No. GTX109939

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all O-GlcNAc transferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

PC-12 , mouse brain

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951060

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Cytoplasm (Potential) , Nucleus

Background

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 8473 OGT
UniProt: O15294 OGT

Research Area

DATA IMAGES

GTX109939 WB Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody (GTX109939) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109939 IP Image

Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody (GTX109939).
Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109939 WB Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
5% SDS-PAGE
O-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109939 ICC/IF Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

GTX109939 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (GTX109939) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX109939 IHC-P Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109939 IHC-P Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109939 IHC-P Image

GTX109939 ICC/IF Image

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

GTX109939 IHC-P Image

REFERENCE

Application Reference

Huang YJ et al. Nanotheranostics 2017; 1 (3) : 326-337 TRAIL-functionalized gold nanoparticles selectively trigger apoptosis in polarized macrophages.

Application : FACS

Reactivity : Human

Sammartino G et al. Biomed Res Int 2016; 2016 : 5713168 Tissue Engineering and Dental Implantology: Biomaterials, New Technologies, and Stem Cells.

Application : WB

Reactivity : Human

Nagai-Okatani C et al. PLoS One 2016; 11 (6) : e0150210 Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.

Application : WB

Reactivity : Rat

Chen YJ et al. Biomed Res Int 2015; 2015 : 924397 Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V.

Application : WB

Reactivity : Human

Ise H et al. Glycobiology 2012; 22 (6) : 788-805 Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.

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