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O-GlcNAc transferase antibody

Overexpression
Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX30868

Cat. No. GTX30868

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all O-GlcNAc transferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IP 2.5-5.0 μg

Note :

IP
using 3.7% formaldehyde-methanol fixation
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

HEK 293T, HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 740-756 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_1952776

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Nucleus,Cytoplasm,Membrane,Cell membrane

Background

O-linked N-acetylglucosamine transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects.

Database

Research Area

DATA IMAGES

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX30868

GTX30868 WB Image

Overexpression

The data was published in the journal Nat Commun in 2016.PMID: 27555448

REFERENCE

Application Reference

Wu JL et al. Nat Commun 2017; 8 (1) : 1854 O-GlcNAcylation is required for B cell homeostasis and antibody responses.
Application : WB
Reactivity : Mouse
Wu JL et al. Nat Commun 2016; 7 : 12526 Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation during apoptosis of activated B cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399