GeneTex
United States (US)

O-GlcNAc transferase antibody

Cat No. GTX30868

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
IP 2.5-5.0 μg

Note :

IP
using 3.7% formaldehyde-methanol fixation

Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

HEK 293T, HeLa nuclear extract
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 740-756 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

O-linked N-acetylglucosamine transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects.

Database

Research Area

REFERENCE
Package List Price ($)
$ 319