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O-GlcNAc transferase antibody

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX32766
Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32766

Cat No. GTX32766

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

Cellular Localization

Nucleus,Cytoplasm,Membrane,Cell membrane

Background

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-O-GlcNAc transferase antibody used in Western Blot (WB). GTX32766

GTX32766 WB Image

WB analysis of various samples using GTX32766 O-GlcNAc transferase antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-O-GlcNAc transferase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32766

GTX32766 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32766 O-GlcNAc transferase antibody.
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 329