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OAT antibody [N1C3]

Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893
Anti-OAT antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103893
Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893
Anti-OAT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103893
Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893

Cat No. GTX103893

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

K562 , THP-1 , Mouse brain , OAT-transfected 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ornithine aminotransferase , GACR , HOGA , OATASE , OKT

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme is the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893

GTX103893 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GTX103893) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OAT antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103893

GTX103893 ICC/IF Image

OAT antibody [N1C3] detects OAT protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: OAT protein stained by OAT antibody [N1C3] (GTX103893) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893

GTX103893 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (GTX103893) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OAT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103893

GTX103893 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using OAT(GTX103893) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OAT antibody [N1C3] used in Western Blot (WB). GTX103893

GTX103893 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103893 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for OAT antibody [N1C3] (GTX103893). Be the first to share your publications with this product.
REVIEW

OAT antibody [N1C3] Cat No. GTX103893

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 10Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169