APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88295-PEP).
Predict Reactivity
Mouse, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NGTSPEIVEKD, from the internal region of the protein sequence according to NP_062818.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier organic anion transporter family member 1B3 , HBLRR , LST-2 , LST-3TM13 , LST3 , OATP-8 , OATP1B3 , OATP8 , SLC21A8
Background
This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of endogenous and xenobiotic compounds and plays a critical role in bile acid and bilirubin transport. Mutations in this gene are a cause of Rotor type hyperbilirubinemia. Alternative splicing of this gene and the use of alternative promoters results in transcript variants encoding different isoforms that differ in their tissue specificity. [provided by RefSeq, Mar 2017]
Database
Research Area
DATA IMAGES
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GTX88295 FACS Image
FACS analysis of PFA-fixed HepG2 cells using GTX88295 OATP8 antibody, Internal.
Blue : Primary antibody
Black : IgG control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX88295 ICC/IF Image
ICC/IF analysis of PFA-fixed A431 cells using GTX88295 OATP8 antibody, Internal.
Green : Primary antibody
Blue : DAP Negative control: Unimmunized goat IgG (10ug/ml)
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX88295 WB Image
WB analysis of HEK293 (A) and A431 (B) cell lysate (35μg protein in RIPA buffer) using GTX88295 OATP8 antibody, Internal.
Dilution : 1μg/ml
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REFERENCE
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REVIEW
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