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OATP8 antibody, Internal

Anti-OATP8 antibody, Internal used in Western Blot (WB). GTX88295
Anti-OATP8 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88295
Anti-OATP8 antibody, Internal used in Flow cytometry (FACS). GTX88295

Cat No. GTX88295

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88295-PEP).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NGTSPEIVEKD, from the internal region of the protein sequence according to NP_062818.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier organic anion transporter family member 1B3 , HBLRR , LST-2 , LST-3TM13 , LST3 , OATP-8 , OATP1B3 , OATP8 , SLC21A8

Background

This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of endogenous and xenobiotic compounds and plays a critical role in bile acid and bilirubin transport. Mutations in this gene are a cause of Rotor type hyperbilirubinemia. Alternative splicing of this gene and the use of alternative promoters results in transcript variants encoding different isoforms that differ in their tissue specificity. [provided by RefSeq, Mar 2017]

Database

DATA IMAGES

Anti-OATP8 antibody, Internal used in Western Blot (WB). GTX88295

GTX88295 WB Image

WB analysis of HEK293 (A) and A431 (B) cell lysate (35μg protein in RIPA buffer) using GTX88295 OATP8 antibody, Internal.
Dilution : 1μg/ml

Anti-OATP8 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88295

GTX88295 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX88295 OATP8 antibody, Internal.
Green : Primary antibody
Blue : DAP Negative control: Unimmunized goat IgG (10ug/ml)
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-OATP8 antibody, Internal used in Flow cytometry (FACS). GTX88295

GTX88295 FACS Image

FACS analysis of PFA-fixed HepG2 cells using GTX88295 OATP8 antibody, Internal.
Blue : Primary antibody
Black : IgG control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339