APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HEK293 Cell Lysate(GTX27902) , Human Spleen Tissue Slide
Product Note
This antibody is specific for OCC-1 N-Terminus
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
OCC-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human OCC-1.The immunogen is located within amino acids 10 - 60 of OCC-1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chromosome 12 open reading frame 75 , AGD3 , OCC-1 , OCC1
Background
OCC-1 (Overexpressed in colon carcinoma-1) was initially identified as a novel human cDNA overexpressed in a colon carcinoma cell line. Elevated levels of OCC-1 mRNA were observed in three of eight colon carcinomas, suggesting that OCC-1 may be a hallmark of only a subset of colon carcinomas. It is highly expressed in skeletal muscle, kidney, placenta, and pancreas tissues, with low to no expression in heart, brain, lung and liver tissues. At least two different OCC-1 mRNA species are known to exist. In vitro transcription and translation of the OCC-1 cDNA yields polypeptides less than 9 kDa. Despite this, OCC-1 from cell lines is detected at ~28 kDa in SDS-PAGE.
Database
Research Area
DATA IMAGES
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GTX85350 WB Image
WB analysis of human spleen tissue lysate using GTX85350 OCC1 antibody.
Working concentration : 2 μg/ml
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GTX85350 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85350 OCC1 antibody.
Working concentration : 20 μg/ml
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GTX85350 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85350 OCC1 antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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