GeneTex
United States (US)

OCT1 antibody

GTX105202_40821_IHC_19011506_227.jpg
GTX105202_42284_20160219_IP_19011506_687.jpg
GTX105202_42284_20160106_IFA_19011506_468.jpg
GTX105202_42284_20151119_WB_19011506_583.jpg
GTX105202_40821_IP_19011506_172.jpg
GTX105202_42284_20160215_IHC-P_R_19011506_339.jpg

Cat No. GTX105202

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OCT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ42836 antibody, OTF1 antibody, POU2F1 antibody, oct-1 antibody, NF-A1 antibody, "POU domain, class 2, transcription factor 1 antibody", octamer-binding protein 1 antibody, octamer-binding transcription factor 1 antibody, OTF-1 antibody, Octamer-binding transcription factor-1 antibody, POU class 2 homeobox 1 antibody

Cellular Localization

Nucleus

Background

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105202_40821_IHC_19011506_227.jpg

GTX105202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using OCT1(GTX105202) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105202_42284_20160219_IP_19011506_687.jpg

GTX105202 IP Image

Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 μg of OCT1 antibody (GTX105202).
Western blot analysis was performed using OCT1 antibody (GTX105202).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105202_42284_20160106_IFA_19011506_468.jpg

GTX105202 ICC/IF Image

OCT1 antibody detects OCT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OCT1 protein stained by OCT1 antibody (GTX105202) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105202_42284_20151119_WB_19011506_583.jpg

GTX105202 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody (GTX105202) diluted at 1:1000.

GTX105202_40821_IP_19011506_172.jpg

GTX105202 IP Image

OCT1 antibody immunoprecipitates OCT1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 μg of OCT1 antibody (GTX105202) 7.5% SDS-PAGE The immunoprecipitated OCT1 protein was detected by OCT1 antibody (GTX105202) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX105202_42284_20160215_IHC-P_R_19011506_339.jpg

GTX105202 IHC-P Image

OCT1 antibody detects OCT1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
OCT1 antibody (GTX105202) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169