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OCT6 antibody

Anti-OCT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX134063
Anti-OCT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134063
Anti-OCT6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134063
Anti-OCT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX134063
Anti-OCT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134063
Anti-OCT6 antibody used in Western Blot (WB). GTX134063

Cat. No. GTX134063

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OCT6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

mouse fetal brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse OCT6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887197

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU domain, class 3, transcription factor 1 , Oct-6 , Oct6 , Otf-6 , Otf6 , Scip , Test1 , Tst-1 , Tst1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-OCT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX134063

GTX134063 IHC-P Image

OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OCT6 stained by OCT6 antibody (GTX134063) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OCT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134063

GTX134063 ICC/IF Image

OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 44840 stained by OCT6 antibody (GTX134063) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-OCT6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134063

GTX134063 IHC-Fr Image

OCT6 antibody detects OCT6 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: OCT6 stained by OCT6 antibody (GTX134063) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-OCT6 antibody used in IHC (Paraffin sections) (IHC-P). GTX134063

GTX134063 IHC-P Image

OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OCT6 stained by OCT6 antibody (GTX134063) diluted at 1:500.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OCT6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134063

GTX134063 ICC/IF Image

OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OCT6 stained by OCT6 antibody (GTX134063) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-OCT6 antibody used in Western Blot (WB). GTX134063

GTX134063 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT6 antibody (GTX134063) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

OCT6 antibody Cat. No. GTX134063

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-OCT6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134063
Date : Anonymous submitted on 02-Sep-2019
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : cerebral cortex
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 6Hr
Figure Note : Rat cerebral cortex. Oct6 (left) and nuclei stained with Hoechst (right).
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169