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ODC antibody [N3C3]

Orthogonal
Anti-ODC antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101521
Anti-ODC antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101521
Anti-ODC antibody [N3C3] used in Western Blot (WB). GTX101521

Cat. No. GTX101521

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ODC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ODC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ornithine decarboxylase 1 , ODC

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ODC antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101521

GTX101521 IHC-P Image

ODC antibody [N3C3] detects ODC protein at cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
ODC antibody [N3C3] (GTX101521) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ODC antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101521

GTX101521 IHC-P Image

ODC antibody [N3C3] detects ODC protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ODC stained by ODC antibody [N3C3] (GTX101521) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ODC antibody [N3C3] used in Western Blot (WB). GTX101521

GTX101521 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ODC antibody [N3C3] (GTX101521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Sekiguchi M et al. NPJ Precis Oncol 2020; 4 : 20 Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169