GeneTex
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ODC antibody [ODC1/485]

Anti-ODC antibody [ODC1/485] used in IHC (Paraffin sections) (IHC-P). GTX34919
Anti-ODC antibody [ODC1/485] used in IHC (Paraffin sections) (IHC-P). GTX34919
Anti-ODC antibody [ODC1/485] used in Flow cytometry (FACS). GTX34919
Anti-ODC antibody [ODC1/485] used in Western Blot (WB). GTX34919

Cat No. GTX34919

Host Mouse
Clonality Monoclonal
Clone Name ODC1/485
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ODC-1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ornithine Decarboxylase 1,Odc,Odc1

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES
Anti-ODC antibody [ODC1/485] used in IHC (Paraffin sections) (IHC-P). GTX34919

GTX34919 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with ODC-1 Monoclonal Antibody (ODC1/485)

Anti-ODC antibody [ODC1/485] used in IHC (Paraffin sections) (IHC-P). GTX34919

GTX34919 IHC-P Image

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ODC-1 Monoclonal Antibody (ODC1/485)

Anti-ODC antibody [ODC1/485] used in Flow cytometry (FACS). GTX34919

GTX34919 FACS Image

Flow Cytometry of human ODC1 on PC3 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ODC1 Monoclonal Antibody (ODC1/485).

Anti-ODC antibody [ODC1/485] used in Western Blot (WB). GTX34919

GTX34919 WB Image

Western Blot of ODC1 in human placental Lysateusing ODC-1 Monoclonal Antibody (ODC1/485).

Package List Price ($)
$ 329