GeneTex
United States (US)

ODC antibody [ODC1/487]

GTX34922_20170712_IHC-P_18121410_751.jpg
GTX34922_20170712_ICCIF_18121410_742.jpg

Cat No. GTX34922

Host Mouse
Clonality Monoclonal
Clone Name ODC1/487
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

ICC/IF:0.5-1ug/ml, IHC-P:0.5-1.0ug/ml for 30 minutes at RT.

Calculated MW

51 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ODC-1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ornithine Decarboxylase 1,Odc,Odc1

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES
GTX34922_20170712_IHC-P_18121410_751.jpg

GTX34922 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/487)

GTX34922_20170712_ICCIF_18121410_742.jpg

GTX34922 ICC/IF Image

IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/487) (Green). DAPI was used to stain the cell nuclei (blue).

Package List Price ($)
$ 329