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OFD1 antibody

Anti-OFD1 antibody used in Western Blot (WB). GTX110010
Anti-OFD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110010
Anti-OFD1 antibody used in Western Blot (WB). GTX110010
Anti-OFD1 antibody used in Western Blot (WB). GTX110010
Anti-OFD1 antibody used in Western Blot (WB). GTX110010

Cat. No. GTX110010

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OFD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OFD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951053

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OFD1 centriole and centriolar satellite protein , 71-7A , CXorf5 , JBTS10 , RP23 , SGBS2

Cellular Localization

Cytoplasm , Centrosome

Background

This gene is located on the X chromosome and encodes a centrosomal protein. A knockout mouse model has been used to study the effect of mutations in this gene. The mouse gene is also located on the X chromosome, however, unlike the human gene it is not subject to X inactivation. Mutations in this gene are associated with oral-facial-digital syndrome type I and Simpson-Golabi-Behmel syndrome type 2. Many pseudogenes have been identified; a single pseudogene is found on chromosome 5 while as many as fifteen have been found on the Y chromosome. Alternatively spliced transcripts have been described for this gene but the biological validity of these transcripts has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OFD1 antibody used in Western Blot (WB). GTX110010

GTX110010 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OFD1 antibody (GTX110010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OFD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110010

GTX110010 IHC-P Image

OFD1 antibody detects OFD1 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
OFD1 antibody (GTX110010) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OFD1 antibody used in Western Blot (WB). GTX110010

GTX110010 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OFD1 antibody (GTX110010) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OFD1 antibody used in Western Blot (WB). GTX110010

GTX110010 WB Image

The data was published in the 2020 in Front Cell Dev Biol. PMID: 32258032

Anti-OFD1 antibody used in Western Blot (WB). GTX110010

GTX110010 WB Image

The data was published in the 2020 in Front Cell Dev Biol. PMID: 32258032

REFERENCE

Application Reference

Casey D Gailey et al. Dev Dyn 2021; 250 (2) : 263-273 Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.
Application : WB
Reactivity : Mouse
Zhang C et al. Front Cell Dev Biol 2020; 8 : 148 Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169