GeneTex
United States (US)

OGDH antibody [9F4BD5]

GTX31117_731_ICC-IF_19011519_584.jpg

Cat No. GTX31117

Host Mouse
Clonality Monoclonal
Clone Name 9F4BD5
Isotype IgG1
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25 μg/ml
ICC/IF 1.0 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

116 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

HEPES buffered saline, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Purified OGDH from porcine heart, purified GLUD from bovine liver.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKGDH,E1k,OGDC

Cellular Localization

Mitochondrion matrix,Nucleus

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES
GTX31117_731_ICC-IF_19011519_584.jpg

GTX31117 ICC/IF Image

ICC/IF analysis of HDFn cells using GTX31117 OGDH antibody [9F4BD5].
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde 20 mins
Permeabilization : Triton X-100 permeabilized (0.1%, 15min)
Dilution : 1 µg/ml overnight at 4°C

Package List Price ($)
$ 319