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OGDH antibody [C2C3], C-term

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124

Cat No. GTX105124

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Huh-7 , Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

oxoglutarate dehydrogenase , AKGDH , E1k , OGDC

Cellular Localization

Mitochondrion matrix

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105124 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
OGDH antibody [C2C3], C-term (GTX105124) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OGDH(GTX105124) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124

GTX105124 ICC/IF Image

OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody (GTX105124) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124

GTX105124 ICC/IF Image

OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169