GeneTex

  • Country / Location Selection

United States (US)

OGDH antibody [C2C3], C-term

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124
Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124
Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

Cat. No. GTX105124

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Product Note

Based on sequence homology, this antibody may cross react with OGDHL protein.

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951054

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oxoglutarate dehydrogenase , AKGDH , E1k , OGDC

Cellular Localization

Mitochondrion matrix

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105124 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody (GTX105124) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

OGDH antibody [C2C3], C-term detects OGDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OGDH antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105124

GTX105124 ICC/IF Image

OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-OGDH antibody [C2C3], C-term used in Western Blot (WB). GTX105124

GTX105124 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

OGDH antibody [C2C3], C-term detects OGDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OGDH(GTX105124) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OGDH antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX105124

GTX105124 IHC-P Image

OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
OGDH antibody [C2C3], C-term (GTX105124) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Atlante S et al. Cell Death Dis 2018; 9 (7) : 756 α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.
Application : WB
Reactivity : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for OGDH antibody [C2C3], C-term (GTX105124). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169