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OGDH antibody

Anti-OGDH antibody used in Western Blot (WB). GTX33374
Anti-OGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX33374
Anti-OGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33374
Anti-OGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX33374

Cat. No. GTX33374

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all OGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887711

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oxoglutarate dehydrogenase , AKGDH , E1k , OGDC

Cellular Localization

Mitochondrion matrix,Nucleus

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-OGDH antibody used in Western Blot (WB). GTX33374

GTX33374 WB Image

WB analysis of various sample lysates using GTX33374 OGDH antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-OGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX33374

GTX33374 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX33374 OGDH antibody.
Dilution : 1:100

Anti-OGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33374

GTX33374 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33374 OGDH antibody.
Blue : DAPI

Anti-OGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX33374

GTX33374 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX33374 OGDH antibody.
Dilution : 1:100

REFERENCE

Application Reference

Cardenas C et al. Sci Signal 2020; 13 (640) Cancer cells with defective oxidative phosphorylation require endoplasmic reticulum-to-mitochondria Ca(2+) transfer for survival.
Leandro J et al. Hum Mol Genet 2020; 29 (7) : 1168-1179 DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.
Green SR et al. Biochim Biophys Acta Proteins Proteom 2020; 1868 (9) : 140448 Regulation of the α-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardson's ground squirrel (Urocitellus richardsonii).
Application : WB
Reactivity : Human
Jo?o Leandro et al. bioRxiv 2019; (Epub) DHTKD1 and OGDH display in vivo substrate overlap and form a hybrid ketoacid dehydrogenase complex.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399