GeneTex
  • Country / Location Selection

United States (US)

OLFML3 antibody [N1C1]

Anti-OLFML3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119826
Anti-OLFML3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX119826
Anti-OLFML3 antibody [N1C1] used in Western Blot (WB). GTX119826

Cat No. GTX119826

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , HCT116

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OLFML3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617595

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

olfactomedin like 3 , HNOEL-iso , OLF44

Cellular Localization

Secreted

Background

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.

Database

Research Area

DATA IMAGES

Anti-OLFML3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119826

GTX119826 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Olfactomedin-like 3(GTX119826) antibody at 1:500 dilution.

Anti-OLFML3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX119826

GTX119826 IHC-P Image

Olfactomedin-like 3 antibody [N1C1] detects Olfactomedin-like 3 protein at cytoplasm in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Olfactomedin-like 3 antibody [N1C1] (GTX119826) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLFML3 antibody [N1C1] used in Western Blot (WB). GTX119826

GTX119826 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OLFML3 antibody [N1C1] (GTX119826) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for OLFML3 antibody [N1C1] (GTX119826). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for OLFML3 antibody [N1C1] (GTX119826). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf