OLFML3 antibody [N1C1]

Cat. No. GTX119826

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all OLFML3 products

Datasheet
Document

View product citations for antibody GTX119826 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OLFML3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617595

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

olfactomedin like 3 , HNOEL-iso , OLF44

Cellular Localization

Secreted

Background

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.

Database

Gene ID: 56944 OLFML3
UniProt: Q9NRN5 OLFML3

Research Area

DATA IMAGES

GTX119826 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with OLFML3 antibody [N1C1] (GTX119826) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX119826 IHC-P Image

Olfactomedin-like 3 antibody [N1C1] detects Olfactomedin-like 3 protein at cytoplasm in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Olfactomedin-like 3 antibody [N1C1] (GTX119826) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX119826 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Olfactomedin-like 3(GTX119826) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty