GeneTex
United States (US)

OLIG1 antibody [C2C3], C-term

GTX104823_39708_20170705_IFA_R_19011506_928.jpg
GTX104823_39708_20151002_IHC-Fr_M_19011506_439.jpg
GTX104823_WB_19011506_893.jpg

Cat No. GTX104823

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human OLIG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Oligodendrocyte Transcription Factor 1,Bhlhb6,Bhlhe21,Olig1

Cellular Localization

Nucleus

Background

Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.

Database

Research Area

DATA IMAGES
GTX104823_39708_20170705_IFA_R_19011506_928.jpg

GTX104823 ICC/IF Image

OLIG1 antibody [C2C3], C-term detects OLIG1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OLIG1 protein stained by OLIG1 antibody [C2C3], C-term (GTX104823) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX104823_39708_20151002_IHC-Fr_M_19011506_439.jpg

GTX104823 IHC-Fr Image

OLIG1 antibody [C2C3] detects OLIG1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. Red: OLIG1 antibody [C2C3] (GTX104823) diluted at 1:200. Blue: DAPI

GTX104823_WB_19011506_893.jpg

GTX104823 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX104823 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169