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OLIG2 antibody [HL1072]

Recombinant antibody
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Frozen sections) (IHC-Fr). GTX636104
Anti-OLIG2 antibody [HL1072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

Cat. No. GTX636104

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1072

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OLIG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oligodendrocyte transcription factor 2 , BHLHB1 , OLIGO2 , PRKCBP2 , RACK17 , bHLHe19

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody [HL1072] used in IHC (Frozen sections) (IHC-Fr). GTX636104

GTX636104 IHC-Fr Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: ice-cold MeOH for 5 min

Anti-OLIG2 antibody [HL1072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636104

GTX636104 ICC/IF Image

Olig2 antibody detects Olig2 protein at oligodendrocyte by immunofluorescent analysis.
Sample:  DIV10 rat cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Olig2 stained by Olig2 antibody (GTX636104) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations