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OLIG2 antibody [HL1072]

Recombinant antibody
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Frozen sections) (IHC-Fr). GTX636104
Anti-OLIG2 antibody [HL1072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104
Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

Cat No. GTX636104

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1072

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909949

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

oligodendrocyte transcription factor 2 , BHLHB1 , OLIGO2 , PRKCBP2 , RACK17 , bHLHe19

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody [HL1072] used in IHC (Frozen sections) (IHC-Fr). GTX636104

GTX636104 IHC-Fr Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: ice-cold MeOH for 5 min

Anti-OLIG2 antibody [HL1072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636104

GTX636104 ICC/IF Image

Olig2 antibody detects Olig2 protein at oligodendrocyte by immunofluorescent analysis.
Sample:  DIV10 rat cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Olig2 stained by Olig2 antibody (GTX636104) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody [HL1072] used in IHC (Paraffin sections) (IHC-P). GTX636104

GTX636104 IHC-P Image

OLIG2 antibody [HL1072] detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody [HL1072] (GTX636104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations