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OLIG2 antibody

Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732
Anti-OLIG2 antibody used in Western Blot (WB). GTX132732
Anti-OLIG2 antibody used in Western Blot (WB). GTX132732
Anti-OLIG2 antibody used in Western Blot (WB). GTX132732

Cat No. GTX132732

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Pig
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

PC3 , U87MG , NE , mouse brain , mouse fetal brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

oligodendrocyte transcription factor 2,BHLHB1,OLIGO2,PRKCBP2,RACK17,bHLHe19

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732

GTX132732 ICC/IF Image

OLIG2 antibody detects OLIG2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OLIG2 protein stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-OLIG2 antibody used in Western Blot (WB). GTX132732

GTX132732 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GTX132732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OLIG2 antibody used in Western Blot (WB). GTX132732

GTX132732 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GTX132732) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-OLIG2 antibody used in Western Blot (WB). GTX132732

GTX132732 WB Image

Various cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OLIG2 antibody (GTX132732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169