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OLIG2 antibody

Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

Cat. No. GTX132732

Reference 6
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399),
25 μl ($169)
See all OLIG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886726

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oligodendrocyte transcription factor 2 , BHLHB1 , OLIGO2 , PRKCBP2 , RACK17 , bHLHe19

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732

GTX132732 ICC/IF Image

OLIG2 antibody detects OLIG2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OLIG2 protein stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kakae M et al. Sci Adv 2023; 9 (29) : eadh0102 The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production.
Chand K.K. et al. bioRxiv 2021; (Epub) Human primed endothelial colony forming cells exert neuroprotective effects in the growth restricted newborn piglet.
Application : IHC-P
Reactivity : Pig
Lee JK et al. J Neuropathol Exp Neurol 2021; 80 (2) : 182-198 Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.
Application : WB
Reactivity : Pig
Barati S et al. Neuropeptides 2019; 75 : 75-84 Effect of mesenchymal stem cells on glial cells population in cuprizone induced demyelination model.
Application : IHC-P
Reactivity : Mouse
Baker EW et al. J Neurotrauma 2018; (Epub) Controlled Cortical Impact Severity Results in Graded Cellular, Tissue, and Functional Responses in a Piglet Traumatic Brain Injury Model.
Application : IHC-P
Reactivity : Pig
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REVIEW

OLIG2 antibody Cat. No. GTX132732

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-OLIG2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132732
Date : Tanusree Sen submitted on 26-Dec-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Brain tissue
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 0.5Hr
Primary Antibody : 1:1:100, 4°C, 12Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169