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OPA1 antibody

Orthogonal
Anti-OPA1 antibody used in Western Blot (WB). GTX48589
Anti-OPA1 antibody used in Western Blot (WB). GTX48589
Anti-OPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48589
Anti-OPA1 antibody used in Western Blot (WB). GTX48589
Anti-OPA1 antibody used in Western Blot (WB). GTX48589
Anti-OPA1 antibody used in Western Blot (WB). GTX48589

Cat. No. GTX48589

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Chicken, Pig, Primate
Package
50 μl ($399)
See all OPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
IHC-P 2.5 μg/ml
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of human OPA1. [Swiss-Prot# O60313]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10623460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OPA1 mitochondrial dynamin like GTPase , BERHS , MGM1 , MTDPS14 , NPG , NTG , largeG

Cellular Localization

Mitochondrion inner membrane

Background

This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OPA1 antibody used in Western Blot (WB). GTX48589

GTX48589 WB Image

WB analysis of MEF cell lysate using GTX48589 OPA1 antibody.

Anti-OPA1 antibody used in Western Blot (WB). GTX48589

GTX48589 WB Image

WB analysis of HeLa, MEF cells, and rat brain tissue lysate using GTX48589 OPA1 antibody.
Dilution : 1 μg/ml

Anti-OPA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48589

GTX48589 IHC-P Image

IHC-P analysis of human prostate tissue using GTX48589 OPA1 antibody.

Anti-OPA1 antibody used in Western Blot (WB). GTX48589

GTX48589 WB Image

WB analysis of post-nuclear extracts of mouse embryonic fibroblast cell lysate using GTX48589 OPA1 antibody.

Anti-OPA1 antibody used in Western Blot (WB). GTX48589

GTX48589 WB Image

Orthogonal

The data was published in the journal PLoS Pathog in 2015.PMID: 26717518

Anti-OPA1 antibody used in Western Blot (WB). GTX48589

GTX48589 WB Image

The data was published in the journal Front Mol Neurosci in 2017. PMID: 28676741

REFERENCE

Application Reference

Chung KP et al. Nat Commun 2019; 10 (1) : 3390 Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis.
Application : WB
Reactivity : Mouse
Pabon MA et al. JCI Insight 2018; 3 (18) Beclin-1 regulates cigarette smoke-induced kidney injury in a murine model of chronic obstructive pulmonary disease.
Application : WB
Reactivity : Mouse
Hsu JY et al. Front Mol Neurosci 2017; 10 : 196 The Truncated C-terminal Fragment of Mutant ATXN3 Disrupts Mitochondria Dynamics in Spinocerebellar Ataxia Type 3 Models.
Application : WB
Reactivity : Mouse
Barbier V et al. Virology 2016; 500 : 149-160 Dengue virus induces mitochondrial elongation through impairment of Drp1-triggered mitochondrial fission.
Application : WB
Reactivity : Human
Cheng CT et al. Cancer Res 2016; (Epub) Metabolic stress-induced phosphorylation of KAP1 Ser473 blocks mitochondrial fusion in breast cancer cells.
Application : WB
Reactivity : Human
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Application Reference
Chung KP et al. Nat Commun 2019; 10 (1):3390 Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis.
Application : WB
Reactivity : Mouse
Pabon MA et al. JCI Insight 2018; 3 (18) Beclin-1 regulates cigarette smoke-induced kidney injury in a murine model of chronic obstructive pulmonary disease.
Application : WB
Reactivity : Mouse
Hsu JY et al. Front Mol Neurosci 2017; 10:196 The Truncated C-terminal Fragment of Mutant ATXN3 Disrupts Mitochondria Dynamics in Spinocerebellar Ataxia Type 3 Models.
Application : WB
Reactivity : Mouse
Barbier V et al. Virology 2016; 500:149-160 Dengue virus induces mitochondrial elongation through impairment of Drp1-triggered mitochondrial fission.
Application : WB
Reactivity : Human
Cheng CT et al. Cancer Res 2016; (Epub) Metabolic stress-induced phosphorylation of KAP1 Ser473 blocks mitochondrial fusion in breast cancer cells.
Application : WB
Reactivity : Human
Yu CY et al. PLoS Pathog 2015; 11 (12):e1005350 Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins.
Ballweg K et al. Am J Physiol Lung Cell Mol Physiol 2014; 307 (11):L895-907 Cigarette smoke extract affects mitochondrial function in alveolar epithelial cells.
Application : WB
Reactivity : Mouse
Yang JY et al. J Mol Cell Cardiol 2013; 59:167-75 Impaired translocation and activation of mitochondrial Akt1 mitigated mitochondrial oxidative phosphorylation Complex V activity in diabetic myocardium.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399