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OPG antibody

Orthogonal
Anti-OPG antibody used in Western Blot (WB). GTX55734
Anti-OPG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55734
Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734
Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734
Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734
Anti-OPG antibody used in Western Blot (WB). GTX55734
Anti-OPG antibody used in Western Blot (WB). GTX55734

Cat. No. GTX55734

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all OPG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887936

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor superfamily member11b , OCIF , OPG , PDB5 , TNFRSF11B , TR1 , Osteoprotegerin , osteoclastogenesis inhibitory factor

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 4982 TNFRSF11B
  • UniProt: O00300 TNFRSF11B

Research Area

DATA IMAGES

Anti-OPG antibody used in Western Blot (WB). GTX55734

GTX55734 WB Image

WB analysis of various sample lysates using GTX55734 OPG antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-OPG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55734

GTX55734 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55734 OPG antibody.
Blue : DAPI

Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734

GTX55734 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX55734 OPG antibody.
Dilution : 1:200

Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734

GTX55734 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55734 OPG antibody.
Dilution : 1:200

Anti-OPG antibody used in IHC (Paraffin sections) (IHC-P). GTX55734

GTX55734 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX55734 OPG antibody.
Dilution : 1:200

Anti-OPG antibody used in Western Blot (WB). GTX55734

GTX55734 WB Image

Orthogonal

The data was published in the 2021 in Microorganisms. PMID: 33805153

Anti-OPG antibody used in Western Blot (WB). GTX55734

GTX55734 WB Image

Orthogonal

The data was published in the 2021 in Microorganisms. PMID: 33805153

REFERENCE

Application Reference

X Zeng et al. Pharmacognosy Magazine 2023; Bee Pollen Stimulated BMP2 and RANK Signaling Pathway in Osteoprogenitors and Improved Bone Mineralization in Rat Model of Femur Fracture
BF Botelho et al. Photomed Laser Surg 2023; 41 (7) : 328-342 Biomodulation of Induced Tooth Movement by Three Methods, Corticopuncture, Photobiomodulation, and Their Combination: An Animal Study
Application : IHC-P
Reactivity : Rat
Zhu X et al. J Orthop Surg Res 2022; 17 (1) : 423 ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription.
Application : WB, IHC-P
Reactivity : Mouse, Rat
Jiah Yeom et al. Microorganisms 2021; 9 (4) Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio.
Application : WB
Reactivity : Human
Wang Z et al. Connect Tissue Res 2020; : 1-16 RANKL expression of primary osteoblasts is enhanced by an IL-17-mediated JAK2/STAT3 pathway through autophagy suppression.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399