ORAI3 antibody

Cat. No. GTX85442

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($399)

See all ORAI3 products

Datasheet
Document

View product citations for antibody GTX85442 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 - 4 μg/mL
ICC/IF	10 μg/mL
IHC-P	2 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180)

Product Note

This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ORAI3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human ORAI3.The immunogen is located within the first 50 amino acids of ORAI3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ORAI calcium release-activated calcium modulator 3 , TMEM142C

Cellular Localization

Membrane

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.

Database

Gene ID: 93129 ORAI3
UniProt: Q9BRQ5 ORAI3

Research Area

DATA IMAGES

GTX85442 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX85442 ORAI3 antibody.
Working concentration : 10 μg/ml

GTX85442 WB Image

WB analysis of A-20 cell lysate using GTX85442 ORAI3 antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml

GTX85442 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX85442 ORAI3 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty