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ORC1 antibody [7F6/1]

Cat. No. GTX20060

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F6/1

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all ORC1 products

APPLICATION

Application Note

Western blotting: use at a concentration of 1 / 1250 - 1 / 2500. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 120 kDa, which corresponds to the predicted molecular weight of ORC 1.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa cells

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

origin recognition complex subunit 1 , HSORC1 , ORC1L , PARC1

Cellular Localization

Nucleus

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399