GeneTex
United States (US)

ORC1 antibody

Cat No. GTX10876

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/200-1/1,000

Note :

WB
Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). A minimum incubation time of 1 hour is recommended with this antibody.

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa cell lysates
PROPERTIES

Form

Liquid

Buffer

Tris buffered saline pH7.3,0.02% Sodium Azide, 0.5% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide: SQDDVLYALKDE (Human).

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Origin Recognition Complex Subunit 1,Hsorc1,Orc1L,Parc1,Orc1

Cellular Localization

Nuclear

Background

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

Package List Price ($)
$ 289