APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89991-PEP).
Product Note
This antibody is expected to recognize all reported isoforms (NP_002543.2; NP_001177810.1; NP_001177811.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DVRQWATSSLSWL, from the C Terminus of the protein sequence according to NP_002543.2; NP_001177810.1; NP_001177811.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
origin recognition complex subunit 4 , ORC4L , ORC4P
Cellular Localization
Nucleus
Background
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
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GTX89991 WB Image
WB analysis of Jurkat nuclear lysate using GTX89991 ORC4L antibody, C-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for ORC4L antibody, C-term (GTX89991). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ORC4L antibody, C-term (GTX89991). Be the first to share your experience with this product.