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ORC4L antibody, C-term

Anti-ORC4L antibody, C-term used in Western Blot (WB). GTX89991

Cat. No. GTX89991

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all ORC4L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89991-PEP).

Product Note

This antibody is expected to recognize all reported isoforms (NP_002543.2; NP_001177810.1; NP_001177811.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DVRQWATSSLSWL, from the C Terminus of the protein sequence according to NP_002543.2; NP_001177810.1; NP_001177811.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

origin recognition complex subunit 4 , ORC4L , ORC4P

Cellular Localization

Nucleus

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-ORC4L antibody, C-term used in Western Blot (WB). GTX89991

GTX89991 WB Image

WB analysis of Jurkat nuclear lysate using GTX89991 ORC4L antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399