GeneTex
United States (US)

ORC4L antibody [C3], C-term

GTX104684_WB_19011506_107.jpg
GTX104684_39820_IFA_19011506_255.jpg

Cat No. GTX104684

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ORC4L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ46668 antibody, ORC4L antibody, ORC4P antibody, ORC4 antibody, origin recognition complex subunit 4 antibody, "origin recognition complex, subunit 4 homolog antibody", "origin recognition complex, subunit 4 antibody"

Cellular Localization

Nucleus

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and -5L. Three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104684_WB_19011506_107.jpg

GTX104684 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
ORC4L antibody
GTX104684 diluted at 1:1000

GTX104684_39820_IFA_19011506_255.jpg

GTX104684 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ORC4L(GTX104684) antibody at 1:500 dilution.

REVIEW

ORC4L antibody [C3], C-term Cat No. GTX104684

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T
Amount used : 30μg
Blocking : 0.5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169