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ORP1 antibody [C3], C-term

Anti-ORP1 antibody [C3], C-term used in Western Blot (WB). GTX107722
Anti-ORP1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107722
Anti-ORP1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107722
Anti-ORP1 antibody [C3], C-term used in Western Blot (WB). GTX107722

Cat. No. GTX107722

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ORP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ORP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037575

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oxysterol binding protein like 1A , ORP-1 , ORP1 , OSBPL1B

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ORP1 antibody [C3], C-term used in Western Blot (WB). GTX107722

GTX107722 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX107722 diluted at 1:1000

Anti-ORP1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107722

GTX107722 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1(GTX107722) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ORP1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107722

GTX107722 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ORP1(GTX107722) antibody at 1:200 dilution.

Anti-ORP1 antibody [C3], C-term used in Western Blot (WB). GTX107722

GTX107722 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ORP1 antibody [C3], C-term (GTX107722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Burkhalter MD et al. J Clin Invest 2019; 129 (7) : 2841-2855 Imbalanced mitochondrial function provokes heterotaxy via aberrant ciliogenesis.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169