GeneTex
United States (US)

ORP1 antibody [C3], C-term

GTX107722_39757_IHC_19011507_229.jpg
GTX107722_WB_19011507_552.jpg
GTX107722_39757_IFA_19011507_812.jpg
GTX107722_39757_WB_M_brain_19011507_619.jpg

Cat No. GTX107722

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , mouse brain

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ORP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Oxysterol Binding Protein Like 1A,Orp-1,Orp1,Osbpl1B,Osbpl1A

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq]

Database

DATA IMAGES
GTX107722_39757_IHC_19011507_229.jpg

GTX107722 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ORP1(GTX107722) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107722_WB_19011507_552.jpg

GTX107722 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX107722 diluted at 1:1000

GTX107722_39757_IFA_19011507_812.jpg

GTX107722 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ORP1(GTX107722) antibody at 1:200 dilution.

GTX107722_39757_WB_M_brain_19011507_619.jpg

GTX107722 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107722 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169