Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ORP150 antibody [HL2006] (GTX637907) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ORP150 antibody [HL2006] (GTX637907) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ORP150 antibody [HL2006] (GTX637907) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.