Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (GTX121273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (GTX121273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Immunofluorescence analysis of methanol-fixed HeLa, using ORP8(GTX121273) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121273 diluted at 1:10000