Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (GTX121273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Unboiled various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (GTX121273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using ORP8(GTX121273) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121273 diluted at 1:10000