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ORP8 antibody

Orthogonal
Anti-ORP8 antibody used in Western Blot (WB). GTX121273
Anti-ORP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121273
Anti-ORP8 antibody used in Western Blot (WB). GTX121273

Cat. No. GTX121273

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ORP8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ORP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oxysterol binding protein like 8 , MST120 , MSTP120 , ORP8 , OSBP10

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ORP8 antibody used in Western Blot (WB). GTX121273

GTX121273 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121273 diluted at 1:10000

Anti-ORP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121273

GTX121273 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ORP8(GTX121273) antibody at 1:500 dilution.

Anti-ORP8 antibody used in Western Blot (WB). GTX121273

GTX121273 WB Image

Orthogonal

Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody (GTX121273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

Application Reference

N Criado Santos et al. Commun Biol 2023; Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites
Nguyen TT et al. Elife 2022; 11 An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion.
Leila Rochin et al. bioRxiv 2019; (Epub) ORP5 Regulates Transport of Lipids and Calcium to Mitochondria at Endoplasmic Reticulum-Mitochondria Membrane Contact Sites.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169