GeneTex
United States (US)

OSBPL6 antibody [C3], C-term

GTX107723_IHC_19011507_766.jpg
GTX107723_39715_20151223_IHC-P_19011507_330.jpg
GTX107723_39715_20160727_IFA_19011507_319.jpg
GTX107723_39715_WB_19011507_593.jpg

Cat No. GTX107723

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human OSBPL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686M06110 antibody, FLJ36583 antibody, FLJ41254 antibody, MGC59642 antibody, ORP6 antibody, OSBPL6 antibody, ORP-6 antibody, OSBP-related protein 6 antibody, oxysterol-binding protein-related protein 6 antibody, oxysterol binding protein-like 6 antibody

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified. [provided by RefSeq]
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Database

DATA IMAGES
GTX107723_IHC_19011507_766.jpg

GTX107723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using OSBPL6 (GTX107723) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107723_39715_20151223_IHC-P_19011507_330.jpg

GTX107723 IHC-P Image

OSBPL6 antibody [C3], C-term detects OSBPL6 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
OSBPL6 antibody [C3], C-term (GTX107723) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107723_39715_20160727_IFA_19011507_319.jpg

GTX107723 ICC/IF Image

OSBPL6 antibody [C3], C-term detects OSBPL6 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OSBPL6 protein stained by OSBPL6 antibody [C3], C-term (GTX107723) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX107723_39715_WB_19011507_593.jpg

GTX107723 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX107723 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169