Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTC antibody (GTX105140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with OTC antibody (GTX105140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC(GTX105140) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min