GeneTex
United States (US)

OTC antibody

GTX105140_39939_IHC_19011506_802.jpg
GTX105140_WB_19011506_778.jpg
GTX105140_39939_WB_M_Liver_19011506_247.jpg

Cat No. GTX105140

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:30000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , HepG2 , mouse Liver

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OTC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ornithine Carbamoyltransferase,Octd,Otc

Cellular Localization

Mitochondrion matrix

Background

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105140_39939_IHC_19011506_802.jpg

GTX105140 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC(GTX105140) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105140_WB_19011506_778.jpg

GTX105140 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX105140 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105140_39939_WB_M_Liver_19011506_247.jpg

GTX105140 WB Image

Sample (20 µg of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX105140 diluted at 1:30000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169