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OTP antibody

Anti-OTP antibody used in IHC (Paraffin sections) (IHC-P). GTX119601
Anti-OTP antibody used in Western Blot (WB). GTX119601

Cat. No. GTX119601

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OTP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OTP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

orthopedia homeobox

Cellular Localization

Nucleus

Background

This gene encodes a member of the homeodomain (HD) family. HD family proteins are helix-turn-helix transcription factors that play key roles in the specification of cell fates. This protein may function during brain development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OTP antibody used in IHC (Paraffin sections) (IHC-P). GTX119601

GTX119601 IHC-P Image

OTP antibody detects OTP protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
OTP antibody (GTX119601) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-OTP antibody used in Western Blot (WB). GTX119601

GTX119601 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX119601 diluted at 1:500

REFERENCE

Application Reference

Shi X et al. Sci Adv 2022; 8 (46) : eabq2987 Hierarchical deployment of Tbx3 dictates the identity of hypothalamic KNDy neurons to control puberty onset.
Rajamani U et al. Cell Stem Cell 2018; 22 (5) : 698-712.e9 Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169