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OTUB1 antibody [AT17E10]

Anti-OTUB1 antibody [AT17E10] used in Western Blot (WB). GTX57636
Anti-OTUB1 antibody [AT17E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57636

Cat. No. GTX57636

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT17E10

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all OTUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT17E10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human OTUB3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OTU deubiquitinase, ubiquitin aldehyde binding 1 , HSPC263 , OTB1 , OTU1 , OTUB1

Cellular Localization

Cytoplasm

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-OTUB1 antibody [AT17E10] used in Western Blot (WB). GTX57636

GTX57636 WB Image

WB analysis of various samples using GTX57636 OTUB1 antibody.
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : 293T whole cell lysate
Loading : 40 μg
Dilution : 1:5000

Anti-OTUB1 antibody [AT17E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57636

GTX57636 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57636 OTUB1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399