GeneTex
United States (US)

OTUB1 antibody, Internal

GTX88681_2176_WB_18122102_490.jpg

Cat No. GTX88681

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88681-PEP).

Predict Reactivity

Human, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-YKEYAEDDNIYQQK, from the internal region of the protein sequence according to NP_060140.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OTU1 Antibody , OTB1 Antibody , HSPC263 Antibody

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88681_2176_WB_18122102_490.jpg

GTX88681 WB Image

WB analysis of mouse brain lysate using GTX88681 OTUB1 antibody, Internal.
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer