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OTUB1 antibody [N1C1]

Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973
Anti-OTUB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101973
Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973
Anti-OTUB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101973

Cat. No. GTX101973

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all OTUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OTUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OTU deubiquitinase, ubiquitin aldehyde binding 1 , HSPC263 , OTB1 , OTU1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973

GTX101973 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] (GTX101973) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-OTUB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101973

GTX101973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1(GTX101973) antibody at 1:50 dilution.

Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973

GTX101973 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] (GTX101973) diluted at 1:500.

Anti-OTUB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101973

GTX101973 IHC-P Image

OTUB1 antibody [N1C1] detects OTUB1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
OTUB1 stained by OTUB1 antibody [N1C1] (GTX101973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169