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OTUB1 antibody [N1C1]

Anti-OTUB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101973
Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973
Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973
Anti-OTUB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101973

Cat No. GTX101973

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.249 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OTUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OTU deubiquitinase, ubiquitin aldehyde binding 1 , HSPC263 , OTB1 , OTU1

Background

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-OTUB1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101973

GTX101973 IHC-P Image

OTUB1 antibody [N1C1] detects OTUB1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
OTUB1 stained by OTUB1 antibody [N1C1] (GTX101973) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973

GTX101973 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX101973 diluted at 1:1000

Anti-OTUB1 antibody [N1C1] used in Western Blot (WB). GTX101973

GTX101973 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with OTUB1 antibody [N1C1] (GTX101973) diluted at 1:500.

Anti-OTUB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101973

GTX101973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1(GTX101973) antibody at 1:50 dilution.

REFERENCE
There are currently no references for OTUB1 antibody [N1C1] (GTX101973). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for OTUB1 antibody [N1C1] (GTX101973). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf