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OTUB2 antibody [11B3]

KO/KD Overexpression
Anti-OTUB2 antibody [11B3] used in Western Blot (WB). GTX83953
Anti-OTUB2 antibody [11B3] used in Western Blot (WB). GTX83953
Anti-OTUB2 antibody [11B3] used in Flow cytometry (FACS). GTX83953

Cat. No. GTX83953

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11B3

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all OTUB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:500
FACS 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:37024477)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OTU deubiquitinase, ubiquitin aldehyde binding 2 , C14orf137 , OTB2 , OTU2

Background

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Database

Research Area

DATA IMAGES

Anti-OTUB2 antibody [11B3] used in Western Blot (WB). GTX83953

GTX83953 WB Image

WB analysis of various cell lines using GTX83953 OTUB2 antibody [11B3].
Loading : 35 ug per lane

Anti-OTUB2 antibody [11B3] used in Western Blot (WB). GTX83953

GTX83953 WB Image

Overexpression

WB analysis of HEK293T cells transfected with OTUB2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83953 OTUB2 antibody [11B3].
Loading : 5 ug per lane

Anti-OTUB2 antibody [11B3] used in Flow cytometry (FACS). GTX83953

GTX83953 FACS Image

FACS analysis of HEK293T cells transfected with either OTUB2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83953 OTUB2 antibody [11B3].

REFERENCE

Application Reference

Zhu Q et al. Signal Transduct Target Ther 2023; 8 (1) : 142 OTUB1 promotes osteoblastic bone formation through stabilizing FGFR2.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519