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OTX2 antibody [1H12G8B2]

Anti-OTX2 antibody [1H12G8B2] used in ELISA (ELISA). GTX60449
Anti-OTX2 antibody [1H12G8B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60449
Anti-OTX2 antibody [1H12G8B2] used in IHC (Paraffin sections) (IHC-P). GTX60449
Anti-OTX2 antibody [1H12G8B2] used in Western Blot (WB). GTX60449
Anti-OTX2 antibody [1H12G8B2] used in IHC (Paraffin sections) (IHC-P). GTX60449
Anti-OTX2 antibody [1H12G8B2] used in Western Blot (WB). GTX60449

Cat. No. GTX60449

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H12G8B2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all OTX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human OTX2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

orthodenticle homeobox 2 , CPHD6 , MCOPS5

Cellular Localization

Nucleus

Background

This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-OTX2 antibody [1H12G8B2] used in ELISA (ELISA). GTX60449

GTX60449 ELISA Image

ELISA analysis of antigen using GTX60449 OTX2 antibody [1H12G8B2].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-OTX2 antibody [1H12G8B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60449

GTX60449 ICC/IF Image

ICC/IF analysis of U251 cells using GTX60449 OTX2 antibody [1H12G8B2].
Green : OTX2
Red: Actin filaments

Anti-OTX2 antibody [1H12G8B2] used in IHC (Paraffin sections) (IHC-P). GTX60449

GTX60449 IHC-P Image

IHC-P analysis of colon tissue (left) and colon cancer tissue (right) using GTX60449 OTX2 antibody [1H12G8B2].

Anti-OTX2 antibody [1H12G8B2] used in Western Blot (WB). GTX60449

GTX60449 WB Image

WB analysis of HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate using GTX60449 OTX2 antibody [1H12G8B2].

Anti-OTX2 antibody [1H12G8B2] used in IHC (Paraffin sections) (IHC-P). GTX60449

GTX60449 IHC-P Image

IHC-P analysis of human stomach tissue (left) and brain tissue (right) using GTX60449 OTX2 antibody [1H12G8B2].

Anti-OTX2 antibody [1H12G8B2] used in Western Blot (WB). GTX60449

GTX60449 WB Image

WB analysis of human OTX2 (AA: 40-297) recombinant protein using GTX60449 OTX2 antibody [1H12G8B2].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409