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OTX2 antibody

Anti-OTX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133210
Anti-OTX2 antibody used in Western Blot (WB). GTX133210
Anti-OTX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133210

Cat No. GTX133210

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Y79
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse OTX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

orthodenticle homeobox 2,E130306E05Rik

Database

Research Area

DATA IMAGES
Anti-OTX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133210

GTX133210 IHC-P Image

OTX2 antibody detects OTX2 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: OTX2 protein stained by OTX2 antibody (GTX133210) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OTX2 antibody used in Western Blot (WB). GTX133210

GTX133210 WB Image

Whole cell extract (37.5 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with OTX2 antibody (GTX133210) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-OTX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133210

GTX133210 IHC-Fr Image

OTX2 antibody detects OTX2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: OTX2 stained by OTX2 antibody (GTX133210) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169