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OVOL2 antibody

KO/KD Orthogonal Overexpression
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

Cat. No. GTX120220

Reference 1
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all OVOL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HCT-116 , HCT-116 nuclear extract , mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse OVOL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625551

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ovo like zinc finger 2 , 1700108N11Rik , 1810007D21Rik , M-OVO , M-OVO-A , M-OVO-B , MOVO , Ovo2 , Zfp339 , movo2

Cellular Localization

Nucleus

Background

DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.

Database

Research Area

DATA IMAGES

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Orthogonal

HCT-116 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (GTX120220) diluted at 1:1000.

Anti-OVOL2 antibody used in Western Blot (WB). GTX120220

GTX120220 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (GTX120220) diluted at 1:500.

REFERENCE

Application Reference

Jeyarajah MJ et al. Cells 2020; 9 (4) The Transcription Factor OVOL2 Represses ID2 and Drives Differentiation of Trophoblast Stem Cells and Placental Development in Mice.
Application : WB, IP, ChIP assay
Reactivity : Mouse
Submit a Reference

REVIEW

OVOL2 antibody Cat. No. GTX120220

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020.PMID: 32244352
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020.PMID: 32244352
Anti-OVOL2 antibody used in Western Blot (WB). GTX120220
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020.PMID: 32244352
Anti-OVOL2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX120220
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cells in 2020.PMID: 32244352
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169