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Obestatin antibody

Anti-Obestatin antibody used in Western Blot (WB). GTX79314
Anti-Obestatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79314

Cat. No. GTX79314

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Obestatin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues F(1) N A P F D V G I K L S G A Q Y Q Q H G R A L(23) of the rat Obestatin gene.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11178088

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ghrelin , 2210006E23Rik , Ghr , MTLRP , MTLRPAP , m46

Cellular Localization

Secreted

Background

This gene encodes a preproprotein that undergoes proteolytic processing to yield two bioactive peptides, ghrelin and obestatin. The hormone ghrelin plays a role in enhancing appetite and has numerous other biological functions that include stimulating the secretion of growth hormone (somatotropin) from the anterior pituitary gland. Obestatin is thought to be a hormone that functions in decreasing appetite. Mice lacking the encoded protein develop normally and exhibit no gross anatomical abnormalities. This gene encodes distinct isoforms, some or all of which may undergo similar proteolytic processing. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-Obestatin antibody used in Western Blot (WB). GTX79314

GTX79314 WB Image

WB analysis of samples using GTX79314 Obestatin antibody.

Anti-Obestatin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79314

GTX79314 ICC/IF Image

ICC/IF analysis of MKN-45 cells using GTX79314 Obestatin antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399