GeneTex
United States (US)

Occludin antibody

GTX114949_40660_20161012_IHC-Fr_R_18121410_486.jpg
GTX114949_40660_IHC_18121410_115.jpg
GTX114949_40660_WB_18121410_497.jpg

Cat No. GTX114949

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.78mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BLCPMG antibody, OCLN antibody, occludin antibody, tight junction protein occludin TM4 minus antibody

Cellular Localization

Membrane; Multi-pass membrane protein , Cell junction , tight junction

Background

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]
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Research Area

DATA IMAGES
GTX114949_40660_20161012_IHC-Fr_R_18121410_486.jpg

GTX114949 IHC-Fr Image

Occludin antibody detects Occludin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Occludin protein stained by Occludin antibody (GTX114949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX114949_40660_IHC_18121410_115.jpg

GTX114949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin(GTX114949) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114949_40660_WB_18121410_497.jpg

GTX114949 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX114949 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
REVIEW

Occludin antibody Cat No. GTX114949

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf