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Occludin antibody

Orthogonal
Anti-Occludin antibody used in Western Blot (WB). GTX114949
Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949
Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

Cat. No. GTX114949

Reference 16
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all Occludin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177242

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

occludin , BLCPMG , PPP1R115 , PTORCH1

Cellular Localization

Cell membrane

Background

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-Occludin antibody used in Western Blot (WB). GTX114949

GTX114949 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Occludin antibody (GTX114949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949

GTX114949 ICC/IF Image

Occludin antibody detects Occludin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Occludin stained by Occludin antibody (GTX114949) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949

GTX114949 IHC-Fr Image

Occludin antibody detects Occludin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Occludin protein stained by Occludin antibody (GTX114949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

GTX114949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin(GTX114949) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Xuehui Fan et al. Front Pharmacol 2022; 13 : 834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Walter Muranyi et al. iScience 2022; 25 (6) : 104383 Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in?vitro model of the choroid plexus.
Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1) : 40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
Application : WB, ICC/IF
Reactivity : Mouse
Cheng YQ et al. Neural Regen Res 2022; 17 (4) : 812-818 CircLphn3 protects the blood-brain barrier in traumatic brain injury.
Application : WB
Reactivity : Mouse
Wu C et al. Free Radic Biol Med 2021; 178 : 271-294 A novel mechanism linking ferroptosis and endoplasmic reticulum stress via the circPtpn14/miR-351-5p/5-LOX signaling in melatonin-mediated treatment of traumatic brain injury.
Reactivity : Mouse
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Submit a Reference

REVIEW

Occludin antibody Cat. No. GTX114949

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Occludin antibody used in Western Blot (WB). GTX114949
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Xuehui Fan et al. Front Pharmacol 2022; 13:834948 S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.
Walter Muranyi et al. iScience 2022; 25 (6):104383 Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in?vitro model of the choroid plexus.
Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1):40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.
Application : WB, ICC/IF
Reactivity : Mouse
Cheng YQ et al. Neural Regen Res 2022; 17 (4):812-818 CircLphn3 protects the blood-brain barrier in traumatic brain injury.
Application : WB
Reactivity : Mouse
Wu C et al. Free Radic Biol Med 2021; 178:271-294 A novel mechanism linking ferroptosis and endoplasmic reticulum stress via the circPtpn14/miR-351-5p/5-LOX signaling in melatonin-mediated treatment of traumatic brain injury.
Reactivity : Mouse
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Petrella C et al. Pharmacol Res 2021; 172:105795 Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis.
Application : WB
Reactivity : Mouse
Li B et al. Food Funct 2021; 12 (12):5524-5538 Beneficial effects of Dendrobium officinale on metabolic hypertensive rats by triggering the enteric-origin SCFA-GPCR43/41 pathway.
Zhang LY et al. Evid Based Complement Alternat Med 2020; 2020:9017854 Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota.
Busatto S et al. J Nanobiotechnology 2020; 18 (1):162 Brain metastases-derived extracellular vesicles induce binding and aggregation of low-density lipoprotein.
Application : WB
Reactivity : Human
Alquisiras-Burgos I et al. Exp Neurol 2020; 330:113353 Resveratrol reduces cerebral edema through inhibition of de novo SUR1 expression induced after focal ischemia.
Application : IHC-Fr
Reactivity : Rat
Sun L et al. Mol Pharm 2020; 17 (3):757-768 Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
Application : ICC/IF
Reactivity : Human
Tian H et al. Adv Healthc Mater 2018;:e1800285 uniform core-shell nanoparticles with thiolated hyaluronic acid coating to enhance oral delivery of *.
Reactivity : Human
Jo H et al. Food Chem Toxicol 2017; 108 (Pt A):203-213 Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Application : WB
Reactivity : Human
He Z et al. Biomaterials 2017; 130:28-41 scalable fabrication of size-controlled chitosan nanoparticles for oral delivery of *.
Application : ICC/IF
Reactivity : Human
Hwang D et al. Biomed Pharmacother 2016; Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169