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Occludin antibody

Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949
Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949
Anti-Occludin antibody used in Western Blot (WB). GTX114949

Cat No. GTX114949

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177242

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

occludin , BLCPMG , PPP1R115 , PTORCH1

Cellular Localization

Cell membrane

Background

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-Occludin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114949

GTX114949 ICC/IF Image

Occludin antibody detects Occludin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Occludin stained by Occludin antibody (GTX114949) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Occludin antibody used in IHC (Frozen sections) (IHC-Fr). GTX114949

GTX114949 IHC-Fr Image

Occludin antibody detects Occludin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Occludin protein stained by Occludin antibody (GTX114949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX114949

GTX114949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin(GTX114949) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Occludin antibody used in Western Blot (WB). GTX114949

GTX114949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Occludin antibody (GTX114949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

Occludin antibody Cat No. GTX114949

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Brain Microvascular Endothelial Cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf