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Occludin antibody

Orthogonal
Anti-Occludin antibody used in Western Blot (WB). GTX85016
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX85016
Anti-Occludin antibody used in Western Blot (WB). GTX85016
Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX85016
Anti-Occludin antibody used in Western Blot (WB). GTX85016

Cat. No. GTX85016

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Occludin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Human Liver Tissue Slide , Human Liver Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN.The immunogen is located within the last 50 amino acids of OCLN.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

occludin , BLCPMG , PPP1R115 , PTORCH1

Cellular Localization

Cell membrane

Background

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Database

Research Area

DATA IMAGES

Anti-Occludin antibody used in Western Blot (WB). GTX85016

GTX85016 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Occludin antibody (GTX85016) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX85016

GTX85016 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody.
Dilution : 5 μg/ml

Anti-Occludin antibody used in Western Blot (WB). GTX85016

GTX85016 WB Image

WB analysis of human liver tissue lysate using GTX85016 Occludin antibody.
Working concentration : 1 μg/ml

Anti-Occludin antibody used in IHC (Paraffin sections) (IHC-P). GTX85016

GTX85016 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85016 Occludin antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-Occludin antibody used in Western Blot (WB). GTX85016

GTX85016 WB Image

Orthogonal

The data was published in the journal Front Cell Infect Microbiol in 2017. PMID: 29124041

REFERENCE

Application Reference

Wei MF et al. Oxid Med Cell Longev 2022; 2022 : 7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
Application : WB, IHC-P
Reactivity : Mouse
Li HL et al. RSC Adv. 2018; 8 : 464-471 Isoastragaloside I suppresses LPS-induced tight junction disruption and monocyte adhesion on bEnd.3 cells via an activating Nrf2 antioxidant defense system.
Application : WB
Reactivity : Mouse
Li HL et al. Front Cell Infect Microbiol 2017; 7 : 455 alteration of gut microbiota and inflammatory cytokine/chemokine profiles in 5-* induced intestinal mucositis.
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399